Selective effects of Datura Stramonium on the granular parallel fibres and purkinje cells of the cerebellum in Wistar rats
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Date
2015
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Publisher
International Journal of Anatomy and Research
Abstract
Introduction: Datura stramonium (DS) is a tropical ubiquitous shrub which is often used to increase intoxication
in some beverages and is also freely used as a hallucinogen. It is a depressant of the central nervous system, yet
commonly smoked in like manner tobacco. The present study investigated changes induced by intoxication with
DS on the purkinje cells and parallel fibres of the cerebellum in Wistar rats to further elucidate the effects of
this drug on cerebellar structure.
Materials and Methods: The study was conducted on both male and female Wistar rats (200-250 g). They were
placed into three batches and four groups were derived from each batch, with eight animals per group. Ethanolic
dried seed extract of DS was diluted in normal saline and administered intraperitoneally (I.P.) at a dose of
750mg/kg and given to the treatment groups: once in batch 1, twice in batch 2, twelve hourly and thrice in batch
3, eight hourly per day respectively for 4 weeks, while the control groups received an equivalent of normal
saline. The rats were euthanized and sections of the cerebellum were histologically processed in all groups.
Silver impregnation stain for degenerating axons and neurons was used to elucidate the actions of DS on
purkinje cells and the parallel fibres of the cerebellum.
Results: The result of IP administration of DS extract (750 mg/kg) given three times daily to the treated rats
showed significant histological changes, which included atrophy of the parallel fibres but no significant
changes in the purkinje cells of the cerebellum.
Conclusions: Intoxication of DS seed as a result of excessive ingestion may have a selective degenerative effect
on the parallel fibres of the granule cells of the cerebellum while the purkinje cells are spared; the implication
being motor dysfunction.
Description
Dare S. Sunday Department of Anatomy, Faculty of Biomedical Sciences, Kampala International University, Uganda.
Keywords
Datura stramonium, Cerebellum, Purkinje cells, Parallel fibres, Atrophy, Intraperitoneal