Department of Physiology
Permanent URI for this collection
Browse
Browsing Department of Physiology by Subject "Lycopene"
Now showing 1 - 2 of 2
Results Per Page
Sort Options
- ItemEffects of lycopene on kidney antioxidant enzyme activities and functions in streptozotocin-induced diabetic Wistar rats(Scientific Publishing Group, 2015) Daniel, EzeEjike; Mohammed, Aliyu; Tanko, Yusuf; Ahmed, AbubakarThe present study assessed the effects of lycopene on kidney antioxidant enzymes activities and functions in streptozotocin-induced diabetic Wistar rats.Diabetes was induced in animals by single intra-peritoneal injection of streptozotocin. Thereafter the animals were randomly assigned into the following groups: Group I and II (Normal control + olive oil and Diabetic control + olive oil)while Group III to VI were treated with (10, 20 and 40 mg/kg of lycopene and 2 mg/kg glibenclamide) respectively. Alltreatments were givenonce daily orally for four weeks. Results obtained showed that blood glucose was significantly (P < 0.05) reduced. MDAconcentration was reduced in kidney tissue, with increased activities of antioxidant enzymes (superoxide dismutase, catalase, glutathione peroxidase) in diabetic animals administered with lycopene when compared with diabetic control group.There was significant (P < 0.05) increase in the level of serum sodium ion and reduction in serum urea level in diabetic rats treated with lycopene when compared with the diabetic control group. Histological findings showed improved renal architecture as reflected by reduced glomerular and tubular necrosisin all treated groups when compared with control group. It can be concluded that lycopene protects against diabetes-induced kidney damage through elevation of endogenous antioxidant enzymes and improved renal dysfunction in diabetic animals.
- ItemHypoglycaemic Effect of Lycopene in Streptozotocin-Induced Diabetic Wistar Rats(British Journal of Medicine & Medical Research, 2015-03-31) Ejike, Daniel EzeAim: The study was designed to investigate the hypoglycaemic potential of lycopene in streptozotocin (STZ)-induced diabetic Wistar rats. Methodology: To achieve this, a total of thirty (30) adult Wistar rats of both sexes were used. The animals were made diabetic by single intraperitoneal injection of freshly prepared (60 mg/kg body weight) of STZ. Diabetes was confirmed by the presence of high blood glucose ≥ 200 after 72 hr. Diabetic animals were divided into six (6) groups (1, 2, 3, 4, 5 and 6) comprising five animals each. Animals in Group 1 (Diabetic control) and Group 2 (Normal control) received 0.5 ml of olive oil, while those in groups 2, 3, 4, 5 and 6 were administered 10, 20, 40 and 2 mg/kg b w of lycopene and glibenclamide respectively orally once daily for a period of four weeks. After the last day of treatments, all animals were sacrificed and blood samples collected and the serum separated for determination of serum insulin concentration. The liver tissue was excised and homogenized in equivalent volumes of phosphate buffer for the determination of hepatic glucokinase enzyme activity. Results: The results obtained showed that lycopene at all doses significantly (P < 0.05) decreased the blood glucose concentration steadily from (431.4±48.84 mg/dL) to (171.1±7.65, 118.4±1.97 and 100.8±6.89 mg/dL) after four weeks of treatment. The Serum insulin level was increased from (3.02±0.24 μIU/mL) to (4.02±0.70, 3.96±1.41 and 5.06±0.96 μIU/mL), but was not significant (P>0.05), when compared with diabetic control animals. The activity of hepatic glucokinase was significantly (P<0.05) increased from (8.78±1.11 ng/mL) to (11.96±0.54, 14.23±0.88 and 15.78±0.27 ng/mL), when compared with diabetic control group. Conclusion: It is therefore, suggested that antidiabetic-activity may be linked to enhanced glucokinase enzyme activity and not due to increased serum insulin level as the elevation was not statistically significant (P > 0.05) when compared with the diabetic control group. It is recommended that, lycopene may be used as a dietary component in controlling sustained hyperglycaemia in diabetes.