The haema to logical and biochemical effects of concomitant administration of cotrimoxazole and ageratum conyzoides (asteraceae) in wistarrats

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Kampala International University. School of Health Sciences
A Research Report Submitted to the School of Pharmacy in Partial Fulfillment of the Requirements for the Award of a Bachelor of Pharmacy Degree of Kampala International University
Introduction: Ageratum conyzoides, a member of the family of"Asteraceace" is increasingly being used in traditional medicine for the treatment of diverse ailments (Abiodun, 2009). Objectives: The objective of this study was to determine the haematological and biochemical effects of concomitant use of cotrimoxazole and Ageratum conyzoides leaf aqueous extract in Wistar Rats. Methods: Extraction of phytochemicals was performed by decoction by boiling in a beaker inside a water bath. After the extraction process, three groups (A, B, C) of rats irrespective of sex were treated as follows. The first group (A) of 5 rats was administered only cotrimoxazole orally (trimethoprim (TMP) (5mg/kg of body weight) and sulphamethoxazole (SMX) (25mg/kg of body weight)) orally 6 hourly for 13 consecutive doses (equivalent to 3 days of conventional Pneumocystis carinii pneumonia dosing of20mglkg/day trimethoprim and 1 OOmg/kg/day sulphamethoxazole), the next group (B) of 5 rats was administered a combination of cotrimoxazole and Ageratum conyzoides orally, the third group( C) of5 animals was treated with only Ageratum conyzoides orally. All the treatments were for three days. (I.e.l3 doses which is the dose of cotrimoxazole that would establish steady state concentration within the stated period). Blood samples were collected from the rats at specific time points at 0 minute immediately after sacrificing the rats, I hour, 6 hours, 12 hours, 24 hours, 48hours and 72 hours after completing the 13 doses. The animals were dissected from the abdominal region; blood was collected from the heart through cardiac puncture using suitable syringe and needle into EDTA vacuitaner tubes for haematological analysis and non-EDTA vacuitaner tubes for biochemical analysis.
Haema, Logical and biochemical effects, Concomitant administration, Cotrimoxazole, Ageratum conyzoides (asteraceae) in wistarrats