Effects of aqueous leaf extract of thunbergia alata (boxer ex sims) on reproductive parameters in male wistar rats
Loading...
Date
2023-05-15
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Kampala International University
Abstract
Thunbergia alata (T. alata) leaves have been documented to enhance sexual activity but the parameters it affects remain unknown. This study was to determine the effect of aqueous leaf extract of T. alata on reproductive parameters in male Wistar rats. Twenty-five male wistar rats (150-200g, n=5) were grouped as follows; Group A:1 mL of distilled water (Negative control), Group B:Sildenafil citrate 25mg/kg b. w. (Positive control), Group C; Aqueous leaf extract of T. alataat a dose of 250mg/kg b. w , Group D ;Aqueous leaf extract of T. alata at a dose of 500mg/kg b. w, Group E; Aqueous leaf extract of T. alata at a dose of 1000mg/kg b. w. These rats were orally administered with different test agents daily for a period of 30days and thereafter sedated with Diethyl ether, sacrificed, and the testis and epididymis removed. Sperm variables examined microscopically, serum sex hormones. Indicated that there was a significant (p = 0.006) increase in the mean testosterone levels of the animals treated with 500 mg/kg and 1000 mg/kg of T. alata as compared to those treated with sildenafil. Significant increases in sperm count were seen in the animals which were treated with 1000 mg/kg of T. alata. Regarding the mean levels of follicle stimulating hormone, the study notes that there was a significant (p <0.001) increase in the mean follicle stimulating hormone levels in the animals which were treated with 500 mg/ kg and 1000 mg/kg. A significant (p = 0.006) and (p = 0.013) increase in sperm motility was noted between the animals treated with 1000 mg/kg of T. alata and the animals that were treated with distilled water and 250 mg/kg of T. alata respectively. The study also noted a significant (p = 0.025) and (p <0.001) increase in sperm viability between the animals treated with 500 mg/ kg and 1000 mg/ kg of T. alata with those that received distilled water. Furthermore, there was also a significant (p = 0.001) and (p = 0.013) increase in sperm viability in the animals treated with 1000 mg/kg of T. alata and those that received sildenafil and 250 mg/ kg of T. alata respectively.
Description
Research dissertation submitted to directorate of postgraduate studies and research in partial fulfillment of the requirements for the award of Masters of Science degree in physiology of Kampala International University