In vitro vaginal probiotic suitability of lactobacillus rhamnosus yoba
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Date
2018-12
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Publisher
Kampala International University. School of Health Sciences
Abstract
Aims: Probiotic Lactobacilli have a great potential to produce antimicrobial compounds that inhibit and control the microbial pathogen growth. The effect of Lactobacillus rhamnosus Yoba on the growth of vaginal pathogens and the effect of vaginal substances (V -wash,KL Y Jelly and Today) on the growth of Lactobacillus rhamnosus Yoba were studied using 2 modified methods.
Methods: Using the modified pour plate technique, the effect of vaginal substances on the growth of L.yoba was determined. Agar well diffusion method was used to determine the inhibitory activity of L.yoba towards clinical isolates of both C. albicans and S. aureus.
Results: The vaginal substances vaginal douche, spermicide and sexual lubricant slightly decreased the Log CFUs/ml of Lactobacillus rhamnosus Yoba from 8.18 to 6.23 Log CFUs/ml with time which was not statistically significant (p> 0.05). Lactobacillus rhamnosus Yoba had no antifungal and antibacterial effect on the growth of clinical isolates of Candida albicans and Staphylococcus aureus respectively
Conclusions and Recommendation: Vaginal substances used in this study had no growth effects against L. yoba. Lactobacillus rhamnosus Yoba had no antifungal and antibacterial effect against test organisms thus its recommended to allow time for fermentation in order to obtainenough probiotics and usage of varying concentrations of L. yoba which could be effective against C. albicans and S. aureus.
Key words: Probiotic suitability L. yoba, vaginal douche, spermicide and sexual lubricant, C. a/bicans and S. aureus.
Description
Research Dissertation Submitted for the Partial Fulfillment for the Award of a Bachelor’s Degree of Pharmacy at Kampala International University Western Campus
Keywords
In vitro vaginal probiotic, Lactobacillus rhamnosus yoba