Isolation and identification of potential probiotic bacteria on surfaces of Oreochromis niloticus and Clarias gariepinus from around Kampala, Uganda

Simple item page
dc.contributor.authorKato Charles Drago
dc.contributor.authorKabarozi, Ruth
dc.contributor.authorMajalija, Samue
dc.contributor.authorTamale, Andrew
dc.contributor.authorSengooba, Asuman
dc.contributor.authorLubowa Musisi, Nathan
dc.date.accessioned2018-09-18T08:37:09Z
dc.date.available2018-09-18T08:37:09Z
dc.date.issued2016
dc.descriptionArticle available full texten_US
dc.description.abstractIncreased fish mortality due to infections has forced most farmers to resort the use of chemotherapeutic agents especially antibiotics. The continued use of these drugs in aquaculture is becoming limited as pathogens develop resistance and infer unpredicted long term public health effects. More research efforts are building to identify alternative disease prevention methods, among which the use of probiotics has been proposed. Therefore, the purpose of this study was to identify potential probiotics on surfaces of tilapia and catfish in areas around Kampala. Tilapia and catfish samples were aseptically collected from selected cages, ponds, tanks and hatcheries around Kampala, including Lake Victoria. The skin of fish was swabbed and then cultured on both general purpose and selective media. Probiotic screening was done using the agar spot method. Results revealed complete growth across all samples. The total microbial load was highest in fish from lakes (1000±9.6×105 cfu) and cages (1001±5.0×105 cfu). In all cases tilapia fish was significantly (p<0.0001) more contaminated than catfish. Out of the three strains of probiotics isolated, onlyLactobacillus spp and Lactococcus spp showed antibacterial activity against pathogenic bacteria. The activity of Lactobacillus spp was significantly high (p< 0.0001) with Streptococcus spp (16.5±0.2mm). Lactobacillus spp inhibited growth of only Proteus spp (5±0.2 mm). Our study shows that Lactobacillus spp and Lactococcus spp isolated from tilapia and catfish possess probiotic activity against a number of pathogenic bacteria. Our findings have significant implications for subsequent probiotic formulation and testing in aquaculture.en_US
dc.identifier.issn1996-0808
dc.identifier.urihttp://hdl.handle.net/20.500.12306/1318
dc.language.isoenen_US
dc.publisherAJMRen_US
dc.subjectProbioticsen_US
dc.subjectOreochromis niloticusen_US
dc.subjectClarias gariepinusen_US
dc.subjectAquacultureen_US
dc.titleIsolation and identification of potential probiotic bacteria on surfaces of Oreochromis niloticus and Clarias gariepinus from around Kampala, Ugandaen_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Isolation and identification of potential bacteria on surfaces of Oreochromis niloticus.pdf
Size:
263.67 KB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Public Health