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Browsing by Author "Kiiza, Haulat"

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    In vitro vaginal probiotic suitability of lactobacillus rhamnosus yoba
    (Kampala International University. School of Health Sciences, 2018-12) Kiiza, Haulat
    Aims: Probiotic Lactobacilli have a great potential to produce antimicrobial compounds that inhibit and control the microbial pathogen growth. The effect of Lactobacillus rhamnosus Yoba on the growth of vaginal pathogens and the effect of vaginal substances (V -wash,KL Y Jelly and Today) on the growth of Lactobacillus rhamnosus Yoba were studied using 2 modified methods. Methods: Using the modified pour plate technique, the effect of vaginal substances on the growth of L.yoba was determined. Agar well diffusion method was used to determine the inhibitory activity of L.yoba towards clinical isolates of both C. albicans and S. aureus. Results: The vaginal substances vaginal douche, spermicide and sexual lubricant slightly decreased the Log CFUs/ml of Lactobacillus rhamnosus Yoba from 8.18 to 6.23 Log CFUs/ml with time which was not statistically significant (p> 0.05). Lactobacillus rhamnosus Yoba had no antifungal and antibacterial effect on the growth of clinical isolates of Candida albicans and Staphylococcus aureus respectively Conclusions and Recommendation: Vaginal substances used in this study had no growth effects against L. yoba. Lactobacillus rhamnosus Yoba had no antifungal and antibacterial effect against test organisms thus its recommended to allow time for fermentation in order to obtainenough probiotics and usage of varying concentrations of L. yoba which could be effective against C. albicans and S. aureus. Key words: Probiotic suitability L. yoba, vaginal douche, spermicide and sexual lubricant, C. a/bicans and S. aureus.

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