Phytochemical screening, quantification, and fractionation of aqueous methanolic extract of spathodea campanulata stem bark
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Date
2012-04
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Kampala international international: School of Health Sciences
Abstract
Plants produce a good deal of secondary metabolites which have benefited mankind in
various ways including treatment of diseases. The present study was carried out to identify
and isolate the different phytochemicals present inS. campanulata stem bark aqueous
methanolic extract, to quantify these phytochemicals, and to identify different fractions of the
extract of S. campanulata. The study carried out was an experimental one, done at Kampala
International University Western Campus (KIU-WC), Bushenyi, Uganda. Result from the
phytochemical studies indicated the presences of flavonoids, tannins, alkaloids, saponins, and
phlobatannins. It is indicated that there was a slight different in the yield of infochemicals in
the aqueous methanolic extract of S. campanulata stem bark. The pecrcentage yields of
phytochemicals were as follows: alkaloids 14.26%, flavonoids 18.22%, tannins 22.70%, and
saponins 36.84%. The mobile phases 4:1 and 1:1 eluted fewer compounds than 1 :4. Since the
mobile phase of hexane/methanol= 1 :4 gave a good separation and eluted more compounds
than the other mobile phases, the plates from this mobile phase were used for analysis.
Spathodea campanulata stem bark aqueous methanolic extract contains tannins, saponins
flavonoids, and alkaloids compounds. Further studies should be done to determine the
therapeutic and/or pharmacological activities of the phytochemicals.
Description
A research project report submitted to the school of pharmacy in partial fulfillment of the requirements of the award of bachelor of pharmacy degree of Kampala international university
Keywords
Pharmacy, Phytochemical screening,, Quantification,, campanulata stem bark, Fractionation of aqueous, Methanolic extract