Evaluation of the Anti-Bacterial Activity of Bioactive Fractions from Ethanol Peel Extract of Ananascomosusagainst Escherichia Coli
Date
2017-12
Authors
Tumuhaise, Vincent
Journal Title
Journal ISSN
Volume Title
Publisher
Kampala International University, School of Health Sciences.
Abstract
Antibacterial resistance is not only increasing the healthcare costs, but also the disease severity
and death rates from certain infections that could have been avoided, prevented or treated by
newer and more effective antibacterial agents. However, the antibacterial activity of bioactive
constituents of the crude peel extract obtained from Ananascomosusis yet to be known. This is
the research gap the present research wants to fill.
l he general aim of the study was to isolate and identify the anti-bacterial bioactive constituents
in the crude ethanol peel extract of Ananascomosus.
;\ suitable amount of Ananascomosuspeels were obtained which were then grinded and
pulverized with the help of ethanol solution to obtain a crude ethanol peel extract of
lmmuscomosus. A solvent extraction process on the crude extract was carried out to obtain Jive
fractions which were then tested for antibacterial activity, phytochemical constituent screening
plus MIC and MBC of the bio-active fractions. Of the five fractions obtained from the
A nwwscomosuspee1 extract, only three fractions showed antibacterial activity against
FscĀ·herichia coli. The extraction of the pulverized Ananascomosus peels with 95% v/v ethanol
gave a percentage yield of 8. 9%. The preliminary phytochemieal screening of the 5% wiv extract
revealed the presence of alkaloids, flavonoids, saponins, tannins, and steroids constituents.
Therel<we Ancmascomosuspee1 extract contains bio-active constituents. The ability of the bioactive
fractions obtained from ethanol peel extract of Ananascomosus to inhibit the growth of
Fscherichia coli indicated the presence of chemical constituents of pharmacological importance.
l n conclusion. Ananascomosuspossesses antibacterial properties against the bacteria Escherichia
rn/i which can be utilized in the cure and prevention of bacterial infectious diseases caused by
J:scherichia coli after standardization.
Description
Research report submitted to Pharmacy School in
Partial Fulfillment of the Requirement for the Award
of a Bachelors Degree in Pharmacy by Kampala
International University -Western Campus.
Keywords
Pharmacy, Anti-Bacterial, Evaluation, Bushenyi District