Antioxidant activity of the Ethanolic extracts of leaves of Amaranth spp and cucurbita spp in drosophila melanogaster

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Kampala international international: School of Health Sciences
Background: Several plants show potent antioxidant and free radical scavenging properties, and their role in preventing oxidative stress-induced diseases such as Diabetes mellitus, Alzheimer's disease, Parkinson's disease, rheumatoid arthritis and many others. Objective: In this study, the antioxidant activity of the ethanol extracts of leaves of Amaranth spp and Cucurbita spp and their ability to protect against oxidative stress in Drosophila mel ana gaster w1 1 18wild type flies was determined. Materials and Methods: Flies cultured on food supplemented with 0.05 and O.lmg/ml of Amaranth spp and Cucurbita spp leaf extracts were assayed for longevity, climbing activity, catalase activity, and oxidative stress resistance according to the established protocols. Scavenging activity of extracts on 2, 2-diphenyl-l-picrylhydrazyl free radical (DPPH) and reducing power activity were equally evaluated. Results: There were significant effects of the extracts on DPPH free radical scavenging activity, catalase activity, climbing activity, longevity and oxidative stress resistance. Cucurbita extract showed no significant difference in climbing activity and catalase activity assay compared to the control. Conclusion: This study shows that the ethanol extracts of leaves of Amaranth spp and Cucurbita spp exhibit varying degrees of protection against free radical induced oxidative stress in Drosophila melanogaster.
A research report submitted in partial fulfillment of the Requirements for the award of a bachelor's degree of Pharmacy of Kampala international university
Pharmacy, Antioxidant activity, Amaranth spp and cucurbita spp, Drosophila melanogaster, Ethanolic extracts leaves