Antibacterial activity of Carica papaya and common antibiotics against methicillin resistant staphylococcus epidermidis isolated from Kampala International University Teaching Hospital wards Surfaces, Bushenyi, Uganda
| dc.contributor.author | Abubakar, Sunusi Adam | |
| dc.date.accessioned | 2019-11-15T07:16:24Z | |
| dc.date.available | 2019-11-15T07:16:24Z | |
| dc.date.issued | 2018-10 | |
| dc.description | A dissertation submitted in partial fulfillment of the requirements for the award of the degree of Master of Science in microbiology of Kampala International University | en_US |
| dc.description.abstract | Siaph;’kwoccu.s epickrmk(Is’ is coagulase-negative staph>lococci that 1requentl~ cau* de~ ice or surger>-associated nosocomial infections worldwide. Methicillin resistant S. epidennidix (MRSIi) have been reported with very serious clinical implications. The antibiotics in clinical use are associated with high resistance levels and non-affordability due to high prices. (‘arica papai a that has been documented to have antimicrobial properties might be able to o{Thr a solution. This study was therefore aimed at determining the antibacterial activity of (‘. papaya and common antibiotics against MRSE isolated from wards surfaces of Kampala International 1.‘niversity [‘caching Ilospital. t‘ganda. S~~ab samples collected from selected ward surfaces were inoculated on Mannitol salt agar 11w isolation of,S epickwn,idlc. The isolates were tested against common antibiotics (Amikacin 3Opg. C’efa,olin 30j.tg. (‘elbxitin 3Ogg. I rimethoprim-sullhmethoxa,ole 25j.tg. (‘iproflo~acin 3Ogg and Gcntamicin 3Ogg) using the disc diffusion method. isolates resistant to (‘efoxitin ~scre subjected .0 C’ papa) a leafand seed crude extracts using agar ‘veil diffusion method. Minimum Inhihiton ,MIC) and Bactericidal concentration (MBC) ofthe C papaya leafand seed crude extracts were ictermined. mecA gene was detected from MRSI! using conventional Polymerase chain reaction. Jut ofthe 363 swab samples analyzed. 112 (30.85%) prevalence ofS. ephiermidis was obtained. 3oth C’ pcq;c(rci leaf and seed crude extracts (methanol and acetone) 4’~hibited antibaeteri,l ictivit> against MRSL with MlC’s and MI3Cs ranges of250 to 31.2mg/mi and 125 to 31.3mg ml 1w leaf and seed extracts respectively. Out of 112 S. epidermidis isolates. II (Q.8°’o) were found ~esistant to (‘efoxitin and all vicre pusiti~e for mecA gene. I his study concludes that £ epIdermicus is present in Kill- ElI wards surfaces. It was resistant to rnmethopnm-sulfamethox~ole (80.4%) and sensitive to Cefazolin (93.8%) and all the 1(9.8%) isolates resistant to Cefoxitin were positive for mecA gene. (‘arica papaya leaf and eec] crude extracts (methanol and acetone) were effective against MRSK. It is therefore recommended that KRJ-TII should use stronger disinfectants such as those containing phenol. ‘iguanides and halogens to decontaminate wards surfaces. In addition. Irimethoprim ulfainethw~aeole should not be prescribed in cases £ epiciermidis is implicated. (‘aricci papa,v 2afand seed crude extracts could be a source ofnovel antibiotics for treatment ofMRSI | en_US |
| dc.identifier.uri | http://hdl.handle.net/20.500.12306/3210 | |
| dc.language.iso | en | en_US |
| dc.publisher | Kampala International University. School of Health Sciences Western Campus | en_US |
| dc.subject | Antibiotics | en_US |
| dc.subject | Methicillin | en_US |
| dc.subject | Staphylococcus epidermidis | en_US |
| dc.subject | Bushenyi, Uganda | en_US |
| dc.title | Antibacterial activity of Carica papaya and common antibiotics against methicillin resistant staphylococcus epidermidis isolated from Kampala International University Teaching Hospital wards Surfaces, Bushenyi, Uganda | en_US |
| dc.type | Other | en_US |