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Title: Antimicrobial Resistance Patterns and Molecular Characterization of Klebsiella pneumoniae in Clinical Isolates at Mbarara Regional Referral Hospital
Authors: Musinguzi, Benson
Turugurwa, Joseph
Mwesigye, James
Kassaza, Kennedy
Byarugaba, Fredrick
Kabanda, Taseera
Keywords: Antimicrobial Resistance Patterns
Molecular Characterization
Klebsiella pneumoniae
Clinical Isolates
Mbarara Regional Referral Hospital
Issue Date: 2019
Publisher: Scientific Research Publishing
Citation: How to cite this paper: Turugurwa, J., Mwesigye, J., Kassaza, K., Byarugaba, F., Kabanda, T. and Musinguzi, B. (2019) Antimicrobial Resistance Patterns and Molecular Characterization of Klebsiella pneumoniae in Clinical Isolates at Mbarara Regional Referral Hospital. Advances in Infectious Diseases, 9, 197-225.
Abstract: Background: Klebsiella pneumoniae is one of the most frequent opportunistic pathogens causing a range of infections and being resistant for beta-lactamases (ESBL) and Carbapenemases. Aim: The aim of the present study was to determine the antimicrobial resistance patterns and molecular characterization establishing the phenotypes and genotypes associated with drug resistance, an antibiogram of genotypically positive isolates for resistance of Klebsiella pneumoniae in clinical isolates at MRRH. Materials and Methods: A laboratory-based descriptive cross-sectional study that was conducted from September 2018 to May 2019 at MRRH. Klebsiella pneumoniae was identified by cultural and biochemical methods. Antibiotic sensitivity test was performed by modified Kirby-Bauer disc diffusion technique. ESBL production in Klebsiella pneumoniae was tested by double-disc synergy test, Carbapenemase production by MHT, Boronic Acid or EDTA test using Meropenem phenotypically and both resistance confirmed genotypically by Multiplex PCR. Results: Out of 1055 clinical isolates, 298 (28.2%) were found positive
Description: Joseph Turugurwa1*, James Mwesigye1, Kennedy Kassaza1, Fredrick Byarugaba1, Taseera Kabanda1, Benson Musinguzi2* 1Department of Microbiology, Faculty of Medicine, Mbarara University of Science and Technology, Mbarara, Uganda 2Department of Medical Laboratory Sciences, School of Allied Health Sciences, Kampala International University, Bushenyi, Uganda
ISSN: ISSN Online: 2164-2656
ISSN Print: 2164-2648
Appears in Collections:Department of Pathology and Microbiology

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