Antibacterial activity of Carica papaya and common antibiotics against methicillin resistant staphylococcus epidermidis isolated from Kampala International University Teaching Hospital wards Surfaces, Bushenyi, Uganda
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Date
2018-10
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Kampala International University. School of Health Sciences Western Campus
Abstract
Siaph;’kwoccu.s epickrmk(Is’ is coagulase-negative staph>lococci that 1requentl~ cau* de~ ice or surger>-associated nosocomial infections worldwide. Methicillin resistant S. epidennidix (MRSIi) have been reported with very serious clinical implications. The antibiotics in clinical use are associated with high resistance levels and non-affordability due to high prices. (‘arica papai a that has been documented to have antimicrobial properties might be able to o{Thr a
solution. This study was therefore aimed at determining the antibacterial activity of (‘. papaya
and common antibiotics against MRSE isolated from wards surfaces of Kampala International 1.‘niversity [‘caching Ilospital. t‘ganda.
S~~ab samples collected from selected ward surfaces were inoculated on Mannitol salt agar 11w
isolation of,S epickwn,idlc. The isolates were tested against common antibiotics (Amikacin 3Opg.
C’efa,olin 30j.tg. (‘elbxitin 3Ogg. I rimethoprim-sullhmethoxa,ole 25j.tg. (‘iproflo~acin 3Ogg and
Gcntamicin 3Ogg) using the disc diffusion method. isolates resistant to (‘efoxitin ~scre subjected .0 C’ papa) a leafand seed crude extracts using agar ‘veil diffusion method. Minimum Inhihiton
,MIC) and Bactericidal concentration (MBC) ofthe C papaya leafand seed crude extracts were ictermined. mecA gene was detected from MRSI! using conventional Polymerase chain reaction.
Jut ofthe 363 swab samples analyzed. 112 (30.85%) prevalence ofS. ephiermidis was obtained.
3oth C’ pcq;c(rci leaf and seed crude extracts (methanol and acetone) 4’~hibited antibaeteri,l
ictivit> against MRSL with MlC’s and MI3Cs ranges of250 to 31.2mg/mi and 125 to 31.3mg ml
1w leaf and seed extracts respectively. Out of 112 S. epidermidis isolates. II (Q.8°’o) were found
~esistant to (‘efoxitin and all vicre pusiti~e for mecA gene.
I his study concludes that £ epIdermicus is present in Kill- ElI wards surfaces. It was resistant to
rnmethopnm-sulfamethox~ole (80.4%) and sensitive to Cefazolin (93.8%) and all the 1(9.8%) isolates resistant to Cefoxitin were positive for mecA gene. (‘arica papaya leaf and
eec] crude extracts (methanol and acetone) were effective against MRSK. It is therefore
recommended that KRJ-TII should use stronger disinfectants such as those containing phenol.
‘iguanides and halogens to decontaminate wards surfaces. In addition. Irimethoprim
ulfainethw~aeole should not be prescribed in cases £ epiciermidis is implicated. (‘aricci papa,v 2afand seed crude extracts could be a source ofnovel antibiotics for treatment ofMRSI
Description
A dissertation submitted in partial fulfillment of the requirements for the award of the degree of Master of Science in microbiology of Kampala International University
Keywords
Antibiotics, Methicillin, Staphylococcus epidermidis, Bushenyi, Uganda